The purpose of the present study was to identify phytochemicals and anti-microbial activity of the coir pith part extract of cassava root. Preliminary Phytochemical screening was carried out for all the three extracts: n-hexane, methanol and ethanol as per the standard methods. Antibacterial activity of methanol crude extract of the coir pith part (M. esculenta) was performed and it was carried out by using agar well diffusion method. The preliminary phytochemical screening shows alkaloids and flavonoids present only on methanol crude extract and tannins, saponins, flavonoids, glycosides steroids, terpenoids and phenols are available on ethanol, methanol and n- hexane crude extracts. The zone of inhibition of methanolic extract of M. esculenta is 13.67 ± 0.58 mm, 16.67 ± 0.57mm and 18.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in P. aeruginosa, respectively, and 12.33 ± 1.53mm, 13 ± 1.00mm and 15.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in S. aureus, respectively. Moreover, the extract also showed moderate antibacterial activity at all concentrations in the range between 12 mm to 18mm on both tested organisms. So, the present study concludes that the coir pith extract anti-nutrients because of the presence of tannins, saponins, steroids and phenols.
| Published in | American Journal of BioScience (Volume 10, Issue 5) |
| DOI | 10.11648/j.ajbio.20221005.14 |
| Page(s) | 180-185 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Anti-Bacterial, Cassava Root, Phytochemical
| [1] | Ani, A. I., Atangwho, I. J., Agiang M. A., and Y. E. Alozie. 2012. Biochemical effects of some cassava (Manihot esculenta, Crantz) traditional Nigerian diets in experimental diabetic rat. International Journal Biochemistry Research, 2 (2): 70-77. |
| [2] | Atehnkng, J., and Adetimirin, V. O. 2006. Exploring the African cassava (Manihot esculenta Crantz) germplasm for somatic embryogenic competence: African J. Biotech., 5 (14): 1324-1329. |
| [3] | Awouafack, MD., Mc Graw, LJ., Gottfried, S., Mbouangouere, R., Tane, P., Spiteller, M., and Eloff, JN. 2013. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosemarobustum (Fabaceae): BMC Complement Altern Med. 13: 1. |
| [4] | Dorman, H. J. D. and Stanley, G. 2000. Antimicrobial agents from plants. J. applied biology, Dol: 10.1046/j.1365-2672.2000.00969.x. |
| [5] | Espina, L., Somolinos, M., Loran, S., Conchello, P., Garcia, D., and Pagan, R. 2011. Chemical composition of commercial citrus fruit essential oils and evaluation of their antimicrobial activity acting alone or in combined processes. Food control, 22 (6), 896-902. |
| [6] | Fernebro J, 2011. Fighting bacterial infections Future treatment options: Drug Resist Update, 14: 125–39. |
| [7] | Ganesh, S., and Vennil, J. J. 2011. Phytochemical Analysis of Acanthus ilicifolius and Avicennia officinalis by GC-MS. Res. J. Phytochem, 5: 60-65. |
| [8] | Idris, S., Rosnah, S., Nor, M. Z. M., mokhtar, M. N. and Abdul Gani, S. S. 2019. Physicochemical composition of different parts of cassava (Manihot esculenta Crantz) plant: Halal products Research institute, 78–84, DOI: //doi.org/10.26656/fr.2019.4(s1). |
| [9] | Jigna, P., and Sumitra, CV. 2007. In-vitro antimicrobial activity and phytochemical analysis of some Indian Medicinal plants. Turk J Biol. 31: 53-58. |
| [10] | Lowy, F., 2003. Antimicrobial resistance: the example of Staphylococcus aureus: J. Clin Invest, 111: 1265–73. |
| [11] | Musa, M. S., Abdelrasool, F. E., Elsheikh, E. A., Ahmed, L. A. M. N., Mahmoud, A. L. E., and Yagi, S. M. 2011. Ethnobotanical study of medicinal plants in the Blue Nile State, South-eastern Sudan. J. Medicinal Plants Research, 5 (17): 4287–4297. |
| [12] | Ojelere, O. O., 2016. Phytochemicals, proximate, mineral element composition and antimicrobial activity of some selected medicinal plant seeds: DOI: 10.13140/RG.2.1.2160.2808. |
| [13] | Okamoto, Y., Suzuki, A., Ueda, ito, C., itoigoawa, H., N, K, 2006. Anti-estrogen activity of prenylated isoflavones from milettia pachycarpa. J. Health. Sci. 52, 186-191. |
| [14] | Okeke, B. C., Jacques, R. J., Bento, F. M., Peralba, M. C., and Camargo, F. A. 2007. Characterization of a polycyclic aromatic hydrocarbon-degrading microbial consortium from a petrochemical sludge land farming site. Bioremediation Journal, 11 (1), 1-11. |
| [15] | Perez, C., Paul, M., Bazerque, P. 1990. An antibiotic assay by the agar-well diffusion method. Acta Biol Med Exp. 15: 113-115. |
| [16] | Prashant, T., Bimlesh, K., Mandeep, K., Gurpreet, K., Haleen, K. 2011. Phytochemical screening and extraction. Inter. pharma. sci. 1: 98-104. |
| [17] | Savithramma, N., Venkateswarlu, P., Suhrulatha, D., Basha, SKM., and Venkataramanadevi, CH. 2011. Studies of Boswelli aovalifoliolata Bal. and Herny - An endemic and endangered medicinal plant: The Biosc. 5: 359-362. |
| [18] | Temesgen, Z., Bakalo, B., and Tamirat, H., 2019. Medicinal, Nutritional and Anti- Nutritional Properties of Cassava (Manihot esculenta). Academic Journal of Nutrition. 8 (3): 34-46. |
| [19] | Westby, A. 2002. Cassava utilization, storage and small-scale processing. Cassava: Biology, production and utilization, 281-300. |
| [20] | Zakaria, Z. A., Khairi, H. M., Somchit M. N., Sulaiman M. R. 2006. The in vitro Antibacterial Activity and Brine Shrimp Toxicity of Manihot esculenta var. Sri Pontian (Euphorbiacea) Extracts. International Journal of Pharmacology, 2 (2): 216-220. |
| [21] | Zenon, M., Lenilson S. Genelane C. Francine F., Marcelo C. 2017. Effect of Tamarindus indica L. And Manihot esculenta Extracts on Antibiotic-resistant Bacteria. Pharmacognosy Res, 9 (2): 195–199. |
APA Style
Fekadu Zema Mena, Yeshanew Fikre Ololo. (2022). Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root. American Journal of BioScience, 10(5), 180-185. https://doi.org/10.11648/j.ajbio.20221005.14
ACS Style
Fekadu Zema Mena; Yeshanew Fikre Ololo. Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root. Am. J. BioScience 2022, 10(5), 180-185. doi: 10.11648/j.ajbio.20221005.14
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ajbio.20221005.14,
author = {Fekadu Zema Mena and Yeshanew Fikre Ololo},
title = {Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root},
journal = {American Journal of BioScience},
volume = {10},
number = {5},
pages = {180-185},
doi = {10.11648/j.ajbio.20221005.14},
url = {https://doi.org/10.11648/j.ajbio.20221005.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20221005.14},
abstract = {The purpose of the present study was to identify phytochemicals and anti-microbial activity of the coir pith part extract of cassava root. Preliminary Phytochemical screening was carried out for all the three extracts: n-hexane, methanol and ethanol as per the standard methods. Antibacterial activity of methanol crude extract of the coir pith part (M. esculenta) was performed and it was carried out by using agar well diffusion method. The preliminary phytochemical screening shows alkaloids and flavonoids present only on methanol crude extract and tannins, saponins, flavonoids, glycosides steroids, terpenoids and phenols are available on ethanol, methanol and n- hexane crude extracts. The zone of inhibition of methanolic extract of M. esculenta is 13.67 ± 0.58 mm, 16.67 ± 0.57mm and 18.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in P. aeruginosa, respectively, and 12.33 ± 1.53mm, 13 ± 1.00mm and 15.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in S. aureus, respectively. Moreover, the extract also showed moderate antibacterial activity at all concentrations in the range between 12 mm to 18mm on both tested organisms. So, the present study concludes that the coir pith extract anti-nutrients because of the presence of tannins, saponins, steroids and phenols.},
year = {2022}
}
TY - JOUR T1 - Identification Phytochemicals and Antibacterial Activity of Coir Pith Part of Cassava Root AU - Fekadu Zema Mena AU - Yeshanew Fikre Ololo Y1 - 2022/10/30 PY - 2022 N1 - https://doi.org/10.11648/j.ajbio.20221005.14 DO - 10.11648/j.ajbio.20221005.14 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 180 EP - 185 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20221005.14 AB - The purpose of the present study was to identify phytochemicals and anti-microbial activity of the coir pith part extract of cassava root. Preliminary Phytochemical screening was carried out for all the three extracts: n-hexane, methanol and ethanol as per the standard methods. Antibacterial activity of methanol crude extract of the coir pith part (M. esculenta) was performed and it was carried out by using agar well diffusion method. The preliminary phytochemical screening shows alkaloids and flavonoids present only on methanol crude extract and tannins, saponins, flavonoids, glycosides steroids, terpenoids and phenols are available on ethanol, methanol and n- hexane crude extracts. The zone of inhibition of methanolic extract of M. esculenta is 13.67 ± 0.58 mm, 16.67 ± 0.57mm and 18.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in P. aeruginosa, respectively, and 12.33 ± 1.53mm, 13 ± 1.00mm and 15.33 ± 0.58mm at concentration of 25mg/ml, 50mg/ml and 100mg/ml in S. aureus, respectively. Moreover, the extract also showed moderate antibacterial activity at all concentrations in the range between 12 mm to 18mm on both tested organisms. So, the present study concludes that the coir pith extract anti-nutrients because of the presence of tannins, saponins, steroids and phenols. VL - 10 IS - 5 ER -
Email: