Saffron (Crocus sativus L.) corms were subjected to gamma irradiation doses; 0,5,15,25 Gy (G1-4) then were planted as annual crop in complete randomized split block design with three replicates. The resultant plants 3 months aged and every month up tile before flowering, were vegitatively sprayed with chitosan solution; 0,50,100,200 mg/L concentrations. Data (C1-4) for growth and yield traits were recorded and subjected to statistic ANDVA, revealed that individual G2, G3 as well as C2, C3, C4 performed positive significant impact on growth traits (plant height, flowers fresh weight, number of flowers, fresh and dry weight of flowers m2) that cause to significant positive impacts in yield traits (main saffron stigmas yield /ha, second medicine – therapeutic metabolites (crocin, picrocrocin, safronal), antioxidant activity and secondary by – product, corms yield/ ha) whilst, G4 attained significant negative impacts in growth and yield traits. Combine applications (G2 C2, G2C3, G2C4), (G3C2, G3C3, G3C4) achieved significant synergistic positive impacts on growth and yield traits. Aside (G4C2, G4C3, G4C4) actuated only significant positive impacts on growth and yield traits and quality, that warrant C2-4 overcoming significant impacts for G4. Overall, results strongly manifest G and /or C reliable strategy, in vivo to enhance main saffron stigmas yield, secondary metabolite (crocin, pircorcrocin, Safronal) and antioxidant activity for saffron plants.
| Published in | Journal of Plant Sciences (Volume 7, Issue 6) |
| DOI | 10.11648/j.jps.20190706.12 |
| Page(s) | 137-143 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Saffron, Gamma Irradiation, Chitosan, Elicitation
| [1] | Shaifali Pal; Yadav, A. K.; Singh, Shubhra Rastogi; Gupta, M. M.; Verma, r. k.; Nagegowda, D. A.; Anirban pal; Shasany. A. K. (2017). Nitrogen treatment enhances sterols and withaferin A through transcriptional activation of jasmonate pathway, WRKY transcription factors, and biosynthesis genes in (Withania somnifera L.) Dual. Protoplasma; 254 (1): 389-399. |
| [2] | Moshe, N. (1999). Saffron Cultivation Saffron (pp. 1–17): CRC Press. Nacoulma, A. P., Compaoré, M., Lorenzi, M., Kiendrebeogo, M., & Nacoulma, O. G. (2012). In vitro Antioxidant and Anti-inflammatory Activities. |
| [3] | Turhan, H., Kahriman, F., Egesel, C. O., Gul, M. K., (2007). The effects of different growing media on flowering and corm formation of saffron (Crocus sativus L.). African Journal of Biotechnology 62328-2332. |
| [4] | Abdullaev FI, Espinosa‑Aguirre JJ. (2004). Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect Prev; 28: 426‑32. |
| [5] | Mzabri I., M. Legsayer, M. Chetouani, A. Aamar, N. Kouddane, A. Boukroute I. Bekkouch and I. Berrichi. 2017. Saffron (Crocus sativus L.) yield parameter assessment of abiotic stressed corms stored in low temperature. J. Mater. Environ. Sci. 8 (10): 3588-3597. |
| [6] | Menia, M., S. Iqbal, R. Zahida, S. Tahir, R. H. Kanth, A. A. Saad and A. Hussian. 2018. Production technology of saffron for enhancing productivity. J. Pharmacognos. Phytochem. 7 (1): 1033-1039. |
| [7] | Moradi, M. M. and S. Turhan. 2017. The importance of Saffron plant in Afghanistan’s agricultura. J. Biol. Environ. Sci. 11 (33): 165-169. |
| [8] | Bagur, M. J.; Alonso Salinas, G. L.; Jimenez-Monreal, A. M.; Chaouqi, S.; Llorens, S.; 2018 Saffron: an old medicinal plant and a potential novel functional food Martinez-Tome, M.; Alonso, G. L. Molecules;. 23 (1): 30. |
| [9] | Lahmas, L., Lamkami. T., Delporte, C., Sikdar, S., Van Antwerpen, P., Saalaoui, F., Megaizzi. V.(2017). Thewaste of saffron erop acheap souree of bioactive compounds. Journal of Functional Foods, 35.341-351. |
| [10] | Hosseinzadeh H, Sadeghnia HR (2005). Safranal, a constituent of Crocus sativus (saffron), attenuated cerebral ischemia induced oxidative damage in rat hippocampus. J Pharm Pharm Sc; 8: 394–399. |
| [11] | Rahmani, A. H.; Khan, A. A.; Aldebasi, Y. H. (2017). Saffron (Crocus sativus) and its active ingredients: role in the prevention and treatment of disease. Pharmacognosy Journal; 9 (6): 873-879. |
| [12] | Khorasanchi, Z.; Shafiee, M.; Kermanshahi, F.; Khazaei, M.; Ryzhikov, M.; Parizadeh, M. R.; Kermanshahi, B.; Ferns, G. A.; Avan, A.; Hassanian, S. M. (2018). Crocus sativus a natural food coloring and flavo ring has potent anti- tumor properties. Phytomedicine;. 43: 21-27. |
| [13] | Rios JL, Recio MC, Giner RM, Menez S. (1996). An update review of saffron and its active constituents. Phyther Res; 10: 189‑93. |
| [14] | Bukhari SI, Pattnaik B, Rayees S, Kaul S, Dhar MK. (2015). Safranal of Crocus sativus L. inhibits inducible nitric oxide synthase and attenuates asthma in a mouse model of asthma. Phytother Res; 29: 617‑27. |
| [15] | Das I, Chakrabarty RN, Das S.(2004). Saffron can prevent chemically induced skin carcinogenesis in swiss albino mice. Asian Pac J Cancer Prev; 5: 70‑6. |
| [16] | Bukhari, S. L.; Lnshah Din; Simmi Grewl; Dhar, M. K. (2018). Antiproliferativ effect saffron and its constituents on different cancerous cell lines. Pharmacognosy Research;. 10 (3): 291-295. |
| [17] | Bathare, S. Z. (2018). Saffron as a functional food and a nutraceutical using saffron and its constituents as the nutraceutics to protect against chronic diseases. Acta Horticulturae; (1200): 201-204. |
| [18] | Jindal, S. K.; Manjit, S. and S. Rekha. (2005): Induced mutations in Senna. (Journal of Arid Legumes; 2 (1): 120-123. |
| [19] | Guo, J.; Han, W. and M. Wang. (2008): Ultraviolet and environmental stresses involving in the induction and regulation of anthocyanin biosynthesis African Journal of Biotechnology; 7 (25): 4966-4972. |
| [20] | Gao MingBo; Zhang, W.; and R. ChengJiang. (2011): Significantly improved taxuyunnanine C production in cell suspension culture of Taxus chinensis by process intensification of repeated elicitation, sucrose feeding, and in situ adsorption. World Journal of Microbiology and Biotechnology; 27 (10): 2271-2279. |
| [21] | Wang–YaNing; Wei–YaHui; Hao–HaoYong; Ji–JingYuan (2007): Advances in the research of resveratrol. Acta Botanica Boreaali Occidentalia Sinica; 27 (4): 852-857. |
| [22] | Misra, N. and S. Mehrota. (2006): Effect of mutagens on production of secondary metabolites in callus cultures of Indian sarsaparilla (Hemidesmus indicus). Horticulture, Environment and Biotechnology; 47 (1): 23-27. |
| [23] | Aggarwal, V.; Shanker, V. and V. Agrawal (2013): In vitro mass production of Stevia rebaudiana Bertoni (an anti-diabetic endangered herb) and assessment of phytotoxicity of different heavy metals on stevioside synthesis. Acta Horticulturae; 972: 121-133. |
| [24] | Rawat, J, M.; Balwant Rawat; Susmita Mishra. (2014). Effect of elicitation on picrtin and picrotoxinin oroduction from in vitro products of kurrooa African journal of Biotechnology; 13 (51): 4612-4619. |
| [25] | Liu, C. and C. XiYu. (2008): Enhancement of phenylethanoid glycosides biosynthesis in cell cultures of Cistanche deserticola by osmotic stress. Plant Cell Reports; 27 (2): 357-362. |
| [26] | Puhi, i.; Sstadler, F. and D. Treutter. (2008): Alteration of flavonoid biosynthesis in youing grapevine (vitis vinifera L.) leaves, flowers and berries induced by the dioxygenase inhibitor prohexadione-ca.j-of-. |
| [27] | Kaskin, N. and B. Kunter. (2009): The effects of callus age, UV irradiation and incubation time on trans – resveratol production in grapevine callus cultures. Tarim – Billileri - Dergist; 15 (1) 9-13. |
| [28] | Swabha Takshak; Agrawal, S. B. (2014). Secondary metabolits and phenylpropanoid pathway enzymes as influenced under supplemental ultraviolet-B radiation in withanis somnifera dunal, an indigenous medicinal plant. Journal of photochemistry and photobiology. B, Biology; 140: 332–343. |
| [29] | Yadegari M. (2017). Effects of Zn, Fe Mn and Cu foliar application on essential oils and morpho-physiological traits of lemon balm (Melissa officinalis L.) Journal of Essentiol Oil-Bearng Plants; 20 (2): 284-495. |
| [30] | Smini ndran, R.; Bincy Baby; Nazeem, P, A. (2014). Genetic transformation in ashwagandha (Withania somnifera (L.) Dunal) for hairy root induction and enhancement of secondary metabolites. Journal of Tropical Agriculture; 52 (1): 39-64. |
| [31] | Munish Sharma; Ashok Ahuja; Rajinder Gupta; Sharada Mallubhotla. (2015). Enhanced bacoside production in shoot cultures of Bacopa monnieri under the influence of abiotic elicitors. Natural product Research; 29 (8): 745-749. |
| [32] | Malekpoor, F., Ghasemi Pirbalouti, A., Salimi, A., (2016). Effect of foliar application of chitosan on morphological and physiological characteristics of basil under reduced irrigation. Res. Crop. 17, 354–359. |
| [33] | Berger LRR, Stamford TCM, Stamford NP (2011) Perspectivaspara o uso da quitosana na Revista Iberoamericana dePolímeros 12: 195-215. agricuiltura. |
| [34] | Kurita K. (1998). Chemistry and application of chitin and chitosan. Polym. Degrad. Stabil. 59: 117–120. |
| [35] | Liu L, Shao Z, Zhang M and Wang Q. (2015). Regulation of carotenoid metabolism in tomato. Mol Plant. 8: 28±39. |
| [36] | Kaya, M.; Mujtaba, M.; Bulut, E.; Akyuz, B.; Zelencova, L.; Sofi, K. (2015). Fluctuation in physicochemical properties of chitins extracted from different body parts of honeybee. Carbohydr. Polym. 132, 9–16. |
| [37] | Kaya, M.; Akyuz, L.; Sargin, I.; Mujtaba, M.; Salaberria, A. M.; Labidi, J.; Cakmak, Y. S.; Koc, B.; Baran, T.; Ceter, T. (2017). Incorporation of sporopollenin enhances acid–base durability, hydrophobicity, and mechanical antifungal and antioxidant properties of chitosan films. J. Ind. Eng. Chem. 47, 236–245. |
| [38] | Chakraborty, M., Karun, A., Mitra, A., (2009). Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera. J. Plant Physiol. 166, 63-71. |
| [39] | Valletta, A., De Angelis, G., Badiali, C., Brasili, E., Miccheli, A., Di Cocco, M. E., Pasqua, G., (2016). Acetic acid acts as an elicitor exerting a chitosan-like effect on xanthone biosynthesis in Hypericum perforatum L. root cultures. Plant Cell Rep. 35 (5), 1009e1020. |
| [40] | Karimi, S., Abbaspour, H., Sinaki, J. M., Makarian, H., (2012). Effects of water deficit and chitosan spraying on osmotic adjustment and soluble protein of cultivars castor bean (Ricinus communis L.). J. Stress Physiol Biochem. 28, 160–169. |
| [41] | Zagzog, O. A.; Gad, M. M.; Hafez, N. K. (2017). Effect of Nano-chitosan on Vegetative Growth, Fruiting and Resistance of Malformation of Mango. Trends Hortic. Res., 6, 673–681. |
| [42] | Swapnil D. Meshram, Rajesh D. Deotale, Kekita H. Chute, Ganesh N. Jadhav and Ganesh A. Padghan (2018). Morpho-physiologlcal and yield responses of soybean to foliar sprays of chitosan and IBA J. Soils and Crops 28 (1) 121-127. |
| [43] | Wu, L. and; Liu, M. (2008). Preparation and properties of chitosan-coated NPK compound fertilizer with controlled-release and water-retention. Carbohydr. Polym. 72, 240–247. |
| [44] | Ahmad, N., Fazal, H., Abbasi, B. H., Rashid, M., Mahmood, T., & Fatma, N. (2010). Efficient regeneration and antioxidant potential in regenerated-tissues of Piper nigrum L. Plant Cell, Tissue and Organ Culture, 102, 129–134. |
| [45] | Manzo A., Panseri S., Bertoni D., Giorgi A. (2015). Economic and qualitative traits of Italian Alps saffron. Journal of Mountain Science, 12 (6), 1542-1550. |
APA Style
Tarek Elsayed Sayed Ahamed. (2019). Bioprospecting Elicitation with Gamma Irradiation Combine with Chitosan to Enhance, Yield Production, Bioactive Secondary Metabolites and Antioxidant Activity for Saffron. Journal of Plant Sciences, 7(6), 137-143. https://doi.org/10.11648/j.jps.20190706.12
ACS Style
Tarek Elsayed Sayed Ahamed. Bioprospecting Elicitation with Gamma Irradiation Combine with Chitosan to Enhance, Yield Production, Bioactive Secondary Metabolites and Antioxidant Activity for Saffron. J. Plant Sci. 2019, 7(6), 137-143. doi: 10.11648/j.jps.20190706.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.jps.20190706.12,
author = {Tarek Elsayed Sayed Ahamed},
title = {Bioprospecting Elicitation with Gamma Irradiation Combine with Chitosan to Enhance, Yield Production, Bioactive Secondary Metabolites and Antioxidant Activity for Saffron},
journal = {Journal of Plant Sciences},
volume = {7},
number = {6},
pages = {137-143},
doi = {10.11648/j.jps.20190706.12},
url = {https://doi.org/10.11648/j.jps.20190706.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20190706.12},
abstract = {Saffron (Crocus sativus L.) corms were subjected to gamma irradiation doses; 0,5,15,25 Gy (G1-4) then were planted as annual crop in complete randomized split block design with three replicates. The resultant plants 3 months aged and every month up tile before flowering, were vegitatively sprayed with chitosan solution; 0,50,100,200 mg/L concentrations. Data (C1-4) for growth and yield traits were recorded and subjected to statistic ANDVA, revealed that individual G2, G3 as well as C2, C3, C4 performed positive significant impact on growth traits (plant height, flowers fresh weight, number of flowers, fresh and dry weight of flowers m2) that cause to significant positive impacts in yield traits (main saffron stigmas yield /ha, second medicine – therapeutic metabolites (crocin, picrocrocin, safronal), antioxidant activity and secondary by – product, corms yield/ ha) whilst, G4 attained significant negative impacts in growth and yield traits. Combine applications (G2 C2, G2C3, G2C4), (G3C2, G3C3, G3C4) achieved significant synergistic positive impacts on growth and yield traits. Aside (G4C2, G4C3, G4C4) actuated only significant positive impacts on growth and yield traits and quality, that warrant C2-4 overcoming significant impacts for G4. Overall, results strongly manifest G and /or C reliable strategy, in vivo to enhance main saffron stigmas yield, secondary metabolite (crocin, pircorcrocin, Safronal) and antioxidant activity for saffron plants.},
year = {2019}
}
TY - JOUR T1 - Bioprospecting Elicitation with Gamma Irradiation Combine with Chitosan to Enhance, Yield Production, Bioactive Secondary Metabolites and Antioxidant Activity for Saffron AU - Tarek Elsayed Sayed Ahamed Y1 - 2019/10/31 PY - 2019 N1 - https://doi.org/10.11648/j.jps.20190706.12 DO - 10.11648/j.jps.20190706.12 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 137 EP - 143 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20190706.12 AB - Saffron (Crocus sativus L.) corms were subjected to gamma irradiation doses; 0,5,15,25 Gy (G1-4) then were planted as annual crop in complete randomized split block design with three replicates. The resultant plants 3 months aged and every month up tile before flowering, were vegitatively sprayed with chitosan solution; 0,50,100,200 mg/L concentrations. Data (C1-4) for growth and yield traits were recorded and subjected to statistic ANDVA, revealed that individual G2, G3 as well as C2, C3, C4 performed positive significant impact on growth traits (plant height, flowers fresh weight, number of flowers, fresh and dry weight of flowers m2) that cause to significant positive impacts in yield traits (main saffron stigmas yield /ha, second medicine – therapeutic metabolites (crocin, picrocrocin, safronal), antioxidant activity and secondary by – product, corms yield/ ha) whilst, G4 attained significant negative impacts in growth and yield traits. Combine applications (G2 C2, G2C3, G2C4), (G3C2, G3C3, G3C4) achieved significant synergistic positive impacts on growth and yield traits. Aside (G4C2, G4C3, G4C4) actuated only significant positive impacts on growth and yield traits and quality, that warrant C2-4 overcoming significant impacts for G4. Overall, results strongly manifest G and /or C reliable strategy, in vivo to enhance main saffron stigmas yield, secondary metabolite (crocin, pircorcrocin, Safronal) and antioxidant activity for saffron plants. VL - 7 IS - 6 ER -
Email: