Effect of Different Carbon Sources on <i>in vitro</i> Shoot Regeneration and Multiplication of <i>Cissampelos pareira</i> (L.) - An Important Medicinal Plant

Cingithalam Munaswamy Reddy Narendra Reddy; Palyam Venkatesh Chaithanya Lakshmi; Varagiri Narayanachetty Swetha Prasuna; Mustrahally Raja Gopal; Gunda Sudhasri Purna; Bathula Srinivas

doi:doi:10.11648/j.jps.20190706.11

Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant

Cingithalam Munaswamy Reddy Narendra Reddy, Palyam Venkatesh Chaithanya Lakshmi, Varagiri Narayanachetty Swetha Prasuna, Mustrahally Raja Gopal, Gunda Sudhasri Purna, Bathula Srinivas

Published in Journal of Plant Sciences (Volume 7, Issue 6)

Received: 24 September 2019 Accepted: 17 October 2019 Published: 28 October 2019

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Abstract

An efficient in vitro protocol for shoot regeneration and production of multiple shoots of Cissampelos pareira (L) was developed. In the present study, the effect of different carbon sources, sucrose, fructose, maltose and glucose was investigated on in vitro shoot regeneration by using axillary bud of Cissampelos pareira (L). The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used. The highest number of shoots (8.06±0.11) was observed on MS medium supplemented with 2% fructose and along with BAP 1.0mg/l and NAA 0.5mg/l and regeneration frequency 95%. The least number of shoots were obtained on MS medium supplemented with 4% maltose and (3.4±0.10) shoots and glucose (3.26±0.11). Among the different carbon sources used in the present study, fructose 2% proved to be a better choice for multiple shoot regeneration followed by Sucrose, maltose, and glucose from axillary bud explants of Cissampelos pareira. In vitro shoots were then excised from the shoot clumps and transferred to the rooting medium containing NAA, IBA (0.5-3.0mg/l). The well-rooted plantlets were then separated from the culture tubes and transferred into sterile soil and vermiculate (1:1) in the greenhouse. Finally, the hardened plants were transferred to the field environment for utmost survivability.

Published in	Journal of Plant Sciences (Volume 7, Issue 6)
DOI	10.11648/j.jps.20190706.11
Page(s)	131-136
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Axillary Bud Explants, Carbon Sources, BAP-6-Benzyl Amino Purine, NAA-1-Napthalene Acetic Acid, Micro Propagation, Cissampelos pareira

References

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Cingithalam Munaswamy Reddy Narendra Reddy, Palyam Venkatesh Chaithanya Lakshmi, Varagiri Narayanachetty Swetha Prasuna, Mustrahally Raja Gopal, Gunda Sudhasri Purna, et al. (2019). Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant. Journal of Plant Sciences, 7(6), 131-136. https://doi.org/10.11648/j.jps.20190706.11

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ACS Style

Cingithalam Munaswamy Reddy Narendra Reddy; Palyam Venkatesh Chaithanya Lakshmi; Varagiri Narayanachetty Swetha Prasuna; Mustrahally Raja Gopal; Gunda Sudhasri Purna, et al. Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant. J. Plant Sci. 2019, 7(6), 131-136. doi: 10.11648/j.jps.20190706.11

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AMA Style

Cingithalam Munaswamy Reddy Narendra Reddy, Palyam Venkatesh Chaithanya Lakshmi, Varagiri Narayanachetty Swetha Prasuna, Mustrahally Raja Gopal, Gunda Sudhasri Purna, et al. Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant. J Plant Sci. 2019;7(6):131-136. doi: 10.11648/j.jps.20190706.11

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@article{10.11648/j.jps.20190706.11,
  author = {Cingithalam Munaswamy Reddy Narendra Reddy and Palyam Venkatesh Chaithanya Lakshmi and Varagiri Narayanachetty Swetha Prasuna and Mustrahally Raja Gopal and Gunda Sudhasri Purna and Bathula Srinivas},
  title = {Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant},
  journal = {Journal of Plant Sciences},
  volume = {7},
  number = {6},
  pages = {131-136},
  doi = {10.11648/j.jps.20190706.11},
  url = {https://doi.org/10.11648/j.jps.20190706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20190706.11},
  abstract = {An efficient in vitro protocol for shoot regeneration and production of multiple shoots of Cissampelos pareira (L) was developed. In the present study, the effect of different carbon sources, sucrose, fructose, maltose and glucose was investigated on in vitro shoot regeneration by using axillary bud of Cissampelos pareira (L). The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used. The highest number of shoots (8.06±0.11) was observed on MS medium supplemented with 2% fructose and along with BAP 1.0mg/l and NAA 0.5mg/l and regeneration frequency 95%. The least number of shoots were obtained on MS medium supplemented with 4% maltose and (3.4±0.10) shoots and glucose (3.26±0.11). Among the different carbon sources used in the present study, fructose 2% proved to be a better choice for multiple shoot regeneration followed by Sucrose, maltose, and glucose from axillary bud explants of Cissampelos pareira. In vitro shoots were then excised from the shoot clumps and transferred to the rooting medium containing NAA, IBA (0.5-3.0mg/l). The well-rooted plantlets were then separated from the culture tubes and transferred into sterile soil and vermiculate (1:1) in the greenhouse. Finally, the hardened plants were transferred to the field environment for utmost survivability.},
 year = {2019}
}

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TY  - JOUR
T1  - Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant
AU  - Cingithalam Munaswamy Reddy Narendra Reddy
AU  - Palyam Venkatesh Chaithanya Lakshmi
AU  - Varagiri Narayanachetty Swetha Prasuna
AU  - Mustrahally Raja Gopal
AU  - Gunda Sudhasri Purna
AU  - Bathula Srinivas
Y1  - 2019/10/28
PY  - 2019
N1  - https://doi.org/10.11648/j.jps.20190706.11
DO  - 10.11648/j.jps.20190706.11
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 131
EP  - 136
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20190706.11
AB  - An efficient in vitro protocol for shoot regeneration and production of multiple shoots of Cissampelos pareira (L) was developed. In the present study, the effect of different carbon sources, sucrose, fructose, maltose and glucose was investigated on in vitro shoot regeneration by using axillary bud of Cissampelos pareira (L). The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used. The highest number of shoots (8.06±0.11) was observed on MS medium supplemented with 2% fructose and along with BAP 1.0mg/l and NAA 0.5mg/l and regeneration frequency 95%. The least number of shoots were obtained on MS medium supplemented with 4% maltose and (3.4±0.10) shoots and glucose (3.26±0.11). Among the different carbon sources used in the present study, fructose 2% proved to be a better choice for multiple shoot regeneration followed by Sucrose, maltose, and glucose from axillary bud explants of Cissampelos pareira. In vitro shoots were then excised from the shoot clumps and transferred to the rooting medium containing NAA, IBA (0.5-3.0mg/l). The well-rooted plantlets were then separated from the culture tubes and transferred into sterile soil and vermiculate (1:1) in the greenhouse. Finally, the hardened plants were transferred to the field environment for utmost survivability.
VL  - 7
IS  - 6
ER  -

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Author Information

Cingithalam Munaswamy Reddy Narendra Reddy

Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Palyam Venkatesh Chaithanya Lakshmi

Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Varagiri Narayanachetty Swetha Prasuna

Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Mustrahally Raja Gopal

Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Gunda Sudhasri Purna

Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Bathula Srinivas

Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India

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Cingithalam Munaswamy Reddy Narendra Reddy, Palyam Venkatesh Chaithanya Lakshmi, Varagiri Narayanachetty Swetha Prasuna, Mustrahally Raja Gopal, Gunda Sudhasri Purna, et al. (2019). Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant. Journal of Plant Sciences, 7(6), 131-136. https://doi.org/10.11648/j.jps.20190706.11

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ACS Style

Cingithalam Munaswamy Reddy Narendra Reddy; Palyam Venkatesh Chaithanya Lakshmi; Varagiri Narayanachetty Swetha Prasuna; Mustrahally Raja Gopal; Gunda Sudhasri Purna, et al. Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant. J. Plant Sci. 2019, 7(6), 131-136. doi: 10.11648/j.jps.20190706.11

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Cingithalam Munaswamy Reddy Narendra Reddy, Palyam Venkatesh Chaithanya Lakshmi, Varagiri Narayanachetty Swetha Prasuna, Mustrahally Raja Gopal, Gunda Sudhasri Purna, et al. Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant. J Plant Sci. 2019;7(6):131-136. doi: 10.11648/j.jps.20190706.11

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@article{10.11648/j.jps.20190706.11,
  author = {Cingithalam Munaswamy Reddy Narendra Reddy and Palyam Venkatesh Chaithanya Lakshmi and Varagiri Narayanachetty Swetha Prasuna and Mustrahally Raja Gopal and Gunda Sudhasri Purna and Bathula Srinivas},
  title = {Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant},
  journal = {Journal of Plant Sciences},
  volume = {7},
  number = {6},
  pages = {131-136},
  doi = {10.11648/j.jps.20190706.11},
  url = {https://doi.org/10.11648/j.jps.20190706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20190706.11},
  abstract = {An efficient in vitro protocol for shoot regeneration and production of multiple shoots of Cissampelos pareira (L) was developed. In the present study, the effect of different carbon sources, sucrose, fructose, maltose and glucose was investigated on in vitro shoot regeneration by using axillary bud of Cissampelos pareira (L). The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used. The highest number of shoots (8.06±0.11) was observed on MS medium supplemented with 2% fructose and along with BAP 1.0mg/l and NAA 0.5mg/l and regeneration frequency 95%. The least number of shoots were obtained on MS medium supplemented with 4% maltose and (3.4±0.10) shoots and glucose (3.26±0.11). Among the different carbon sources used in the present study, fructose 2% proved to be a better choice for multiple shoot regeneration followed by Sucrose, maltose, and glucose from axillary bud explants of Cissampelos pareira. In vitro shoots were then excised from the shoot clumps and transferred to the rooting medium containing NAA, IBA (0.5-3.0mg/l). The well-rooted plantlets were then separated from the culture tubes and transferred into sterile soil and vermiculate (1:1) in the greenhouse. Finally, the hardened plants were transferred to the field environment for utmost survivability.},
 year = {2019}
}

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TY  - JOUR
T1  - Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant
AU  - Cingithalam Munaswamy Reddy Narendra Reddy
AU  - Palyam Venkatesh Chaithanya Lakshmi
AU  - Varagiri Narayanachetty Swetha Prasuna
AU  - Mustrahally Raja Gopal
AU  - Gunda Sudhasri Purna
AU  - Bathula Srinivas
Y1  - 2019/10/28
PY  - 2019
N1  - https://doi.org/10.11648/j.jps.20190706.11
DO  - 10.11648/j.jps.20190706.11
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 131
EP  - 136
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20190706.11
AB  - An efficient in vitro protocol for shoot regeneration and production of multiple shoots of Cissampelos pareira (L) was developed. In the present study, the effect of different carbon sources, sucrose, fructose, maltose and glucose was investigated on in vitro shoot regeneration by using axillary bud of Cissampelos pareira (L). The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used. The highest number of shoots (8.06±0.11) was observed on MS medium supplemented with 2% fructose and along with BAP 1.0mg/l and NAA 0.5mg/l and regeneration frequency 95%. The least number of shoots were obtained on MS medium supplemented with 4% maltose and (3.4±0.10) shoots and glucose (3.26±0.11). Among the different carbon sources used in the present study, fructose 2% proved to be a better choice for multiple shoot regeneration followed by Sucrose, maltose, and glucose from axillary bud explants of Cissampelos pareira. In vitro shoots were then excised from the shoot clumps and transferred to the rooting medium containing NAA, IBA (0.5-3.0mg/l). The well-rooted plantlets were then separated from the culture tubes and transferred into sterile soil and vermiculate (1:1) in the greenhouse. Finally, the hardened plants were transferred to the field environment for utmost survivability.
VL  - 7
IS  - 6
ER  -

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